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After that, they were stored at −80 ☌ until the time of RNA extraction. After slaughter, Longissimus thoracis muscle samples were taken and placed immediately into liquid nitrogen for cryopreservation. For ingredients, chemical composition, and feeding methods of the four diets administered, for the manner and timing (in relation to the weight of the animals) of pig slaughtering, and for regulations on the protection of animals at slaughter, refer to. In the middle of the trial, in the experimental group D4, a pig died of natural causes.

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The subjects were all fed a standard diet until the start of the trial, after which each group was given its respective diet, which was a standard diet for growing-finishing pigs (D1) the same diet as D1, enriched with extruded linseed, an n-3 PUFAs source (D2) the same diet as D2, enriched with vitamin E and selenium (D3) and the same diet as D2, enriched with grape skin and oregano extracts, sources of natural polyphenols (D4). The 48 pigs were divided into four experimental groups of 12 animals, each balanced for weight, father, and sex. A functional enrichment analysis of DEGs, using Cytoscape, revealed that the diet enriched with extruded linseed and supplemented with vitamin E and selenium promoted a more rapid and massive immune system response because the overall function of muscle tissue was improved, while those enriched with extruded linseed and supplemented with grape skin and oregano extracts promoted the presence and oxidative stability of n-3 PUFAs, increasing the anti-inflammatory potential of the muscular tissue. Using a published RNA-seq dataset and a selective workflow, the study aimed to find the differences in gene expression and investigate how differentially expressed genes (DEGs) were implicated in the cellular composition and metabolism of muscle tissue of 48 Italian Large White pigs under different dietary conditions. However, to date, the functionality of these components within muscle tissue is not well understood. The addition of n-3 polyunsaturated fatty acids ( n-3 PUFAs) to the swine diet increases their content in muscle cells, and the additional supplementation of antioxidants promotes their oxidative stability. This can determine the high quality of the muscle tissue as regards the lipid composition consequently, the meat will be qualitatively better for human health. From the results of the expression analysis, it was possible to deduce that, in the diets, the oxidative stability of the n-3 fatty acids increased, consistent with an increase in the fluidity of cell membranes, and increasing the anti-inflammatory potential of muscle.

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For this reason, our study aimed to investigate the differences in gene expression of the Longissimus thoracis muscle of Italian Large White pigs fed with four different diets: a standard diet for growing-finishing pigs and three experimental diets one supplemented with extruded linseed, a source of omega-3 polyunsaturated fatty acids, another with extruded linseed plus vitamin E and selenium as antioxidants, and another with extruded linseed plus oregano and grape skin extracts, which are natural polyphenols. In pigs, on the other hand, there are few studies dealing with the use of omega-3 polyunsaturated fatty acids in the diet. Volker, 2016, Graph libraries – Cytoscape.js with Cola.In humans, a dietary intake of omega-3 polyunsaturated fatty acids along with antioxidants has been shown to have anti-inflammatory and antioxidant activities.Joseph Stahl, Getting started with Cytoscape.js,.I would like to try to test a Jspwiki plugin I would like to develop a JSPWiki plugin to use cytospace.js easily on this wiki system. Get cytoscape.js and include this java script library in your html as source of script.

#Cytoscape cola how to#

I would like to study how to use the library here.

#Cytoscape cola software#

The product became a business, it should be welcomed but I've not been able to use that software easily. Previously I liked TouchGraph as open source network visualization tool.